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basic fibroblast growth factor  (R&D Systems)


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    R&D Systems basic fibroblast growth factor
    Basic Fibroblast Growth Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 905 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+basic+fibroblast+growth+factor+bfgf/Recombinant+Human+FGF+basic%2FFGF2%2FbFGF+(146+aa)+Protein/pmc13132546-182-33-37
    Average 96 stars, based on 905 article reviews
    basic fibroblast growth factor - by Bioz Stars, 2026-10
    96/100 stars

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    Recombinant:

    Article Title: Comparative Cytotoxicity of Inorganic Arsenite and Methylarsenite in Human Brain Cells.
    Article Snippet: For culturing the human mesencephalon-derived cell line LUHMES (ATCC® CRL2927TM), flasks and plates were pre-coated with 50 μg/mL poly-L-ornithine (R&D Systems, Minneapolis, MN) and 1 μg/mL fibronectin (ThermoFisher) according to ATCC’s protocols. .. Undifferentiated LUHMES cells were maintained in proliferation medium consisting of DMEM/F12 (Gibco), 1X N2 supplement (Invitrogen, Carlsbad, CA), and 40 ng/mL recombinant human basic fibroblast growth factor (bFGF) (R&D Systems). .. When cells reached 70% confluency, culture medium was exchanged to the differentiation medium consisting of DMEM/F12, 1X N2 supplement, 2.25 μM tetracycline (Sigma-Aldrich), 1 mM 3’, 5’-cyclic adenosine monophosphate (cAMP) (ACROS Organics, Morris Plains, NJ), and 2 ng/mL recombinant human glial cellderived neurotrophic factor (GDNF) (Invitrogen).

    Article Title: Regulation of Corneal Stromal Cell Behavior by Modulating Curvature Using a Hydraulically Controlled Organ Chip Array
    Article Snippet: The quiescent corneal keratocytes were cultured in serum-starved medium containing DMEM/F12 (11320033, Gibco, USA) supplemented with 1% penicillin/streptomycin (15140122, Gibco, USA), 1 mM L-ascorbic acid 2-phosphate (49752, Sigma-Aldrich, USA), and 1% insulin transferrin selenium solution (I3146, Sigma-Aldrich, USA). .. To transform the keratocytes into activated corneal fibroblasts, DMEM/F12 supplemented with 10% FBS, 1% penicillin/streptomycin, and 10 ng/mL recombinant human basic fibroblast growth factor (bFGF) (233-FB, R&D Systems, USA) was used to maintain the fibroblastic phenotype. .. Finally, to obtain cornea myofibroblasts, the fibroblasts were differentiated using a medium containing DMEM/F12, 10% FBS, and 10 ng/mL recombinant human transforming growth factor (TGF-β) (240-B002, R&D Systems, USA).

    Article Title: RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization
    Article Snippet: .. Cells were cultured at a density of 10,000 cells/well in serum-free DMEM/ F12 (1:1) medium (HyClone, SH30272.02), supplemented with 1% P/S, 20 ng/mL recombinant human epidermal growth factor (EGF) (Sigma), 10 ng/mL recombinant human basic fibroblast growth factor (bFGF) (R&D Systems) and B27 (1:50, Gibco). ..

    Article Title: Isolation, culture, and use of primary murine myoblasts in small-molecule screens
    Article Snippet: Horse serum , Thermo Fisher Scientific , 26-050-088. .. Recombinant human basic fibroblast growth factor (bFGF) , R and D Systems , 233-FB-025. .. bFGF Reconstitution Buffer 2 , R and D Systems , RB02.

    Article Title: Lipoic acid enhances survival of transplanted neural stem cells by reducing transplantation-associated injury
    Article Snippet: The basic media was modified with N-2 Supplement, which included 25 μg/mL bovine insulin (Sigma), 100 μg/mL human transferrin (Sigma), 100 μM putrescine (Sigma), 20 nM progesterone (Sigma), and 30 nM sodium selenite (Sigma). .. Growth media was further supplemented with 20 ng/mL recombinant human epidermal growth factor (R&D Systems, Minneapolis, MN, USA), 20 ng/mL recombinant human basic fibroblast growth factor (bFGF) (R&D Systems), 5 μg/mL heparin (Sigma), and 10 ng/mL recombinant human leukemia inhibitory factor (Chemicon, Temecula, CA, USA). ..

    Article Title: Nuclear Receptor NR5A2 Promotes Neuronal Identity in the Adult Hippocampus.
    Article Snippet: Neurogenesis in the dentate gyrus (DG) of the adult hippocampus is actively involved in brain homeostasis.. Thus, identification of novel regulators in adult neurogenesis could significantly contribute to new therapies.. We have recently unraveled the regulatory role of NR5A2 (also known as LRH1), a druggable orphan nuclear receptor, in embryonic neurogenesis.

    Article Title: RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization.
    Article Snippet: .. Cells were cultured at a density of 10,000 cells/well in serum-free DMEM/ F12 (1:1) medium (HyClone, SH30272.02), supplemented with 1% P/S, 20 ng/mL recombinant human epidermal growth factor (EGF) (Sigma), 10 ng/mL recombinant human basic fibroblast growth factor (bFGF) (R&D Systems) and B27 (1:50, Gibco). ..

    Article Title: Graphene nanoplatelets enhance neuronal differentiation of human bone marrow mesenchymal stem cells
    Article Snippet: The neuron differentiation components, including IBMX (3-isobutyl-1-methylxanthine), B27 supplement, dibutyryl cyclic AMP (dcAMP), and hEGF, were sourced from Sigma Aldrich (Taufkirchen, Germany), while fibroblast growth factor-8 (FGF-8) was obtained from PeproTech (Waltham, USA). .. Nerve growth factor (NGF), recombinant human basic fibroblast growth factor (BFGF), and recombinant human brain-derived neurotrophic factor (BDNF) were purchased from R&D systems (Minnesota, USA). .. Antibodies and conjugates, including Coralite488-conjugated goat anti-rabbit (#SA00013-2), Cy3-conjugated Affinipure goat anti-rabbit (#SA00009-1), MAP2 polyclonal antibody (#17490-1-AP), Nestin polyclonal antibody (#19483-1-AP), and TUBB3 monoclonal antibody (#66375-1-Ig), were sourced from Protein Tech (Chicago, USA).

    Cell Culture:

    Article Title: RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization
    Article Snippet: .. Cells were cultured at a density of 10,000 cells/well in serum-free DMEM/ F12 (1:1) medium (HyClone, SH30272.02), supplemented with 1% P/S, 20 ng/mL recombinant human epidermal growth factor (EGF) (Sigma), 10 ng/mL recombinant human basic fibroblast growth factor (bFGF) (R&D Systems) and B27 (1:50, Gibco). ..

    Article Title: RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization.
    Article Snippet: .. Cells were cultured at a density of 10,000 cells/well in serum-free DMEM/ F12 (1:1) medium (HyClone, SH30272.02), supplemented with 1% P/S, 20 ng/mL recombinant human epidermal growth factor (EGF) (Sigma), 10 ng/mL recombinant human basic fibroblast growth factor (bFGF) (R&D Systems) and B27 (1:50, Gibco). ..



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